文章基本信息

标题：BONE REPAIR WITH DIFFERENTIATED OSTEOBLASTS FROM ADIPOSE DERIVED STEM CELLS IN HYDROXYAPATITE/TRICALCIUM PHOSPHATE IN VIVO
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作者：HASHEMIBENI BATOOL ; DEHGHANI LEILA ; SADEGHI FARZANEH 等
期刊名称：INTERNATIONAL JOURNAL OF PREVENTIVE MEDICINE
印刷版ISSN：2008-7802
出版年度：2016
卷号：7
期号：4
页码：0-0
语种：English
出版社：ISFAHAN UNIVERSITY OF MEDICAL SCIENCES
摘要：Background: Recently, tissue engineering has developed approaches for repair and restoration of damaged skeletal system based on different scaffolds and cells. This study evaluated the ability of differentiated osteoblasts from adipose?derived stem cells (ADSCs) seeded into hydroxyapatite/ tricalcium phosphate (HA?TCP) to repair bone.
Methods: In this study, ADSCs of 6 canines were seeded in HA?TCP and differentiated into osteoblasts in osteogenic mediumin vitro and bone markers evaluated by reverse transcription polymerase chain reaction (RT?PCR). Scanning electron microscopy (SEM) was applied for detection of cells in the pores of scaffold. HA-TCP with differentiated cells as the test group and without cells as the cell?free group were implanted in separate defected sites of canine’s tibia.
After 8 weeks, specimens were evaluated by histological, immunohistochemical methods, and densitometry test. The data were analyzed using the SPSS 18 version software.
Results: The expression of Type I collagen and osteocalcin genes in differentiated cells were indicated by RT?PCR. SEM results revealed the adhesion of cells in scaffold pores. Formation of trabecular bone confirmed by histological sections that revealed the thickness of bone trabecular was more in the test group. Production of osteopontin in extracellular matrix was indicated in both groups. Densitometry method indicated that strength in the test group was similar to cell?free group and natural bone (P>0.05).
Conclusions: This research suggests that ADSCs?derived osteoblasts in HA?TCP could be used for bone tissue engineering and repairing.
关键词：ADIPOSE-DERIVED STEM CELLS; ANIMAL MODEL; BONE REPAIR; HYDROXYAPATITE/TRICALCIUM PHOSPHATE