文章基本信息

标题：Screening, Optimization and Application of Extracellular Phytase from Bacillus Megaterium Isolated from Poultry Waste
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作者：Dhiraj Kumar ; Subramani Rajesh ; Pannerselvam Balashanmugam 等
期刊名称：Journal of Modern Biotechnology
印刷版ISSN：2319-6645
出版年度：2013
卷号：2
期号：2
页码：46-52
出版社：Madras Institute of Biotechnology
摘要：Soil phosphatases play a major role in the mineralization processes of organic phosphorus. A phytase or myo-inositolhexakisphosphate phosphohydrolase (E.C. 3.1.3.8) effectively catalyzes the release of phosphate from phytate andphosphorylated compound. Phytase is derived from different sources such as bacteria and yeasts. The extracellular phytaseproducing bacteria was isolated from poultry and phytase production was screened. It was purified by ammonium sulphatefractionation of protein and dialysis followed by SDS-PAGE. The DNA from Bacillus megaterium was isolated usingCTAB method. The maximum enzyme production was obtained in the 60th h at 37°C, pH 6.5 and 200 rpm shaking. Thebest carbon and nitrogen source was lactose 138 μg/mL and ammonium sulphate 124 μg/mL, respectively. The 16S rRNAgene sequencing was carried out for identification of positive bacteria. Pot experiment was carried out with Zea mays forphosphate utilization and accessed by root and maximum measurement was observed.
关键词：Poultry waste; Phytase; Bacillus megaterium; Plant growth.