文章基本信息

标题：In silico Structural Determination of Fatty acid Hydrolysis and Synthesizing Enzymes for Biofuel Production in Microalgae
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作者：S. Sathya ; S. Meenakshi ; S. Srisudha 等
期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2013
卷号：2
期号：10
页码：5274
出版社：S&S Publications
摘要：Twenty five sequences of algae were retrieved from NCBI for lipase, fatty acid synthase and glycerol 3-phosphate dehydrogenase. Physicochemical parameters were determined using pepstats, ProtParam, ProtScale andNetwork protein sequence analysis. Conserved regions were identified by multiple sequence alignment using Clustal W.The primary structure analysis of three enzymes indicates that the percentage of Methionine residues is approximately4% among the selected microalgae. Secondary structure analysis revealed that the proteins are largely alpha helical; thiswas supported by the homology modeling of three enzymes of Chlamydomonas reinhardtii (L2), Osterococcuslucimarnus (F7) and Osterococcus taurii (G7). Structural validation tools viz., Ramachandran Plot, VERIFY 3D andERRAT indicates the overall accuracy of the developed models. More active sites are present in Osterococcuslucimarnus (F7) than Chlamydomonas reinhardtii (L2) and Osterococcus taurii (G7). The results revealed that thestructural models developed from this study will give useful information for biofuel production.
关键词：Biofuel; Lipase; Fatty acid synthase; Glycerol 3 phosphate dehydrogenase.