文章基本信息

标题：Correlation of Nitric oxide and testicular activity in laboratory mouse, Mus musculus
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作者：VINEETPRAKASH SINGH ; CHANDRA MOHINI CHATURVEDI
期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2013
卷号：2
期号：3
页码：721
出版社：S&S Publications
摘要：This study helps us to understand the interrelationship between the signaling molecule nitric oxide (NO) andtesticular activity. In the first part of the experiment, NO activity (nitrite and nitrate concentration; in the plasma andtestes and NOS (nitric oxide synthase) expression; in testis) was analyzed in sexually immature and mature laboratorymale mouse, Musmusculus. In the second experiment immature mice were administered with NOS inhibitor N-nitro-largininemethyl ester (L-NAME, 1 mg/100 g body weight) and mature with NO donor sodium nitroprusside (SNP, 0.25mg/100 g body weight) intraperitoneally for 7 days. In the third experiment, both immature and mature mice wereadministered with testosterone propionate (TP) for 7 days intramuscularly. Results indicate increased NO level andintense expression of ir-NOS (immunoreactive NOS) in sexually immature mice compared to mature. Although NOSinhibitor has no effect on plasma testosterone level of immature mice; NO donor has suppressed testosterone in maturemice. Further, testosterone injection decreased NO level in both the age groups of mice. It is concluded that NO andgonadal activity exhibit inverse relationship not only in control/ basal condition but experimental induction of increasedNO level may suppress gonadal activity while exogenous testosterone may suppress NO activity. These findings notonly suggest the inverse NO-gonadal relationships but also indicate that modulation of NO activity may affect gonadalactivity and vice versa under different physiological / experimental conditions.
关键词：Nitric oxide; L-NAME; SNP; ir- NOS; Testosterone propionate