文章基本信息

标题：Intracellular redox state regulates the resistance of Zymomonas mobilis alcohol dehydrogenase II to cyanide and oxygen
本地全文：下载
作者：Uldis Kalnenieks ; Ņina Galiņina ; Reinis Rutkis 等
期刊名称：Environmental and Experimental Biology
印刷版ISSN：1691-8088
出版年度：2005
页码：71-81
出版社：University of Latvia
摘要：The variable sensitivity of Zymomonas mobilis iron-containing alcohol dehydrogenase isoenzyme(ADH II) to cyanide and oxygen was studied. Th e cyanide-sensitivity of ADH II was highest incells grown under conditions of vigorous aeration, in which intracellular NADH concentrationwas low. Anaerobically grown bacteria, as well as those cultivated aerobically in the presence ofcyanide, maintained a higher intracellular NADH pool along with a more cyanide-resistant ADH II.In aerobically grown permeabilized cells, cyanide caused gradual inhibition of ADH II, which waslargely prevented by externally added NADH, and, to lesser extent, by NAD. It was demonstratedthat cyanide acted as a competitive inhibitor of ADH II, competing with nicotinamide nucleotides.NADH increased both the cyanide resistance and oxygen resistance of ADH II.
关键词：alcohol dehydrogenase; cyanide resistance; oxygen resistance; Zymomonas mobilis.