文章基本信息

标题：Subcellular localisation of internally deleted HBV core proteins
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作者：Rūta Brūvere ; Sabine Braun ; Anna Zajakina 等
期刊名称：Environmental and Experimental Biology
印刷版ISSN：1691-8088
出版年度：2004
页码：53-64
出版社：University of Latvia
摘要：Hepatitis B virus (HBV) variants with core internal deletions were isolated from renal graftrecipients in whom an association with end stage liver disease was observed. The aim of this workwas evaluation of intracellular localisation of these HBV core variants with internal deletions.Infection of baby hamster kidney cell culture BHC-21 C13 with recombinant Semliki Forest virusesencoding the deleted variant and undeleted wild type HBV core (HBc) genes led to the detectionof HBc protein within the nucleus only in the case of deletion variants. Deleted HBc protein in allprobability may be partially unfolded, hence unstable. As a consequence, deleted HBc proteinsare not part of HBV nucleocapsids, but are left unassembled and are able to cross the nuclearmembrane. We suggest that the presence of deleted HBc proteins within the cell nucleus may be ofpathogenic importance.
关键词：deletion mutants; hepatitis B virus; HBV core protein; Semliki Forest virus; subcellular;localisation.