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标题：Pengaruh Iradiasi Sinar-X terhadap Produksi Antibodi Mencit Galur Balb/c dengan Pemberian Vaksin Toksoid Tetanus
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作者：ERNA WIDYASARI ; SHANTI LISTYAWATI ; ARTINI PANGASTUTI 等
期刊名称：Bioteknologi
印刷版ISSN：0216-6887
出版年度：2007
卷号：4
期号：1
页码：13-19
出版社：UNS Solo
摘要：X-irradiation is a kind of immunosupression which dangerous for health.X-ray can disturb limfosit’s capability to cleavage then reduce the infectionimmune response. Decreasing immune response, caused by X-irradiation,can be shown in the decrease of antibodies production. The aims of thisresearch were to know significant difference’s antibodies productionamong the doses of 0.0 Gy, 0.5 Gy, 1.5 Gy, and 3.0 Gy X-ray and the effect ofX-irradiation on the BALB/c mice antibodies production after tetanus toxoidimmunization. Mice were grouped into four groups with the treatments of0.0 Gy dose, 0.5 Gy dose, 1.5 Gy dose, 3.0 Gy dose X-ray for 1st, 2nd, 3rd, and4th group respectively. External irradiation was applied once on the wholebody of mice. Tetanus toxoid immunization was done 24 hours after Xirradiation.Indirect ELISA was used to determine the presence ofantibodies in serum samples as the quantitative data. Its were analyzed byone way ANOVA and regression. One way of ANOVA was done to knowsignificant difference’s antibodies production among the doses of 0.0 Gy,0.5 Gy, 1.5 Gy, and 3.0 Gy X-ray then continued with DMRT on 5%significant rate if there was any marked difference. Regression was done toknow the effect of X-irradiation on the BALB/c mice antibodies productionafter tetanus toxoid immunization. Qualitative observations were done onpoisoned symptom, the changes of physical and behavior, and mortality.The result of the research showed that there weren’t significant difference’santibodies production with tetanus toxoid vaccine gift among the dose of0.0 Gy, 0.5 Gy and 1.5 Gy as the same as 0.5 Gy, 1,5 Gy, and 3,0 Gy dose ofX-ray. There was a significant difference at 3.0 Gy and 0.0 Gy dose of X-ray.X-irradiation influenced the antibodies production with tetanus toxoidvaccine gift by caused antibody rate degradation along with the increasingof X-ray dose.
关键词：X-ray irradiation; antibodies; BALB/c mice; tetanus toxoid