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标题：Seleksi dan Identifikasi Bakteri Alkalifilik Penghasil Xilanase dari Tanah Bukit Krakitan, Bayat, Klaten
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作者：HANIFAH THOYIB ; RATNA SETYANINGSIH ; SURANTO 等
期刊名称：Bioteknologi
印刷版ISSN：0216-6887
出版年度：2007
卷号：4
期号：1
页码：6-12
出版社：UNS Solo
摘要：Xylanase producing alkaliphilic bacteria are bacteria that can grow well inalkaline environment. They can produce xylanase that optimum at high pHto hydrolyze xylans to be xylooligosaccharides and xylose. The calcareoussoil with pH more than 7,3 can be xylanase producing alkaliphilic bacterianature habitat. The aims of this research were (1) to isolate xylanaseproducing alkaliphilic bacteria isolated from calcareous soil in limestonehill area Krakitan, Bayat, Klaten and (2) to determine the kind of xylanaseproducing alkaliphilic bacteria which has high xylanolytic activity. Forisolation and selection of xylanase producing alkaliphilic bacteria, thealkaline medium pH 9-10 were used, and 0,5 % oat spelt xylan was added.The calcareous soil was taken from limestone hill area and then was filteredand inoculated on to alkaline medium. After that, they were incubated inshaker incubator for 3 days at 150 rpm in 30-37°C. Then, the enrichmentsample was grown in the same medium, that 1,8% agar was added. Thecolonies that grew and produced clearing zone which had the highestxylanolytic activity were chosen then characterized and identified. Thegrowth ability isolates were examined in xylan medium at pH 7, 8, 9, 10, and11 in order to decide bacteria of fakultative or obligate alkaliphilic.Xylanase producing alkaliphilic bacteria could be isolated from calcareoussoil in limestone hill area Krakitan, Bayat, Klaten. The identified 10xylanase producing alkaliphilic bacteria that had xylanolytic activity werePseudomonas HT-1a, Pseudomonas HT-1b, Bacillus HT-2a, Bacillus HT-2d,Flavobacterium rigense HT-3c, Micrococcus luteus HT-3d, Paracoccusalcaliphilus HT-5c, Alcaligenes HT-4c, Alcaligenes HT-5a, and AlcaligenesHT-5b.
关键词：selection; identification; xylanase producing alkaliphilic;bacteria; soil.