Mobile phones may act as a reservoir of pathogen which in turn may cause significant loss in the form of morbidity and mortality. The distribution pattern of pathogens varies with sampling site and also depends on mode of transmission. The organisms isolated from cell phones causes many infections. But some organisms developed resistance against some antibiotics. The total of 472 swab samples of mobile phone from hospital personnel were collected and studied for the presence of pathogens. Bacterial growth was obtained from 98.7% (466/472) of the mobile phones from hospital population. Staphylococcus sp. showed maximum growth 63.7% (297/466), Staphylococcus sp. was observed resistant to six antibiotics out of twelve antibiotics that we check, while it was sensitive to ciprofloxacin and ofloxacin (96.2%), roxithromycin, tetracycline and pefloxacin (93.6%) and gentamicine (54.8%).