Aim of the present study is prevalence and biochemical characterization of bacteria isolated from some River/Canal bank water sources, door of transport vehicles and shop-counters. Water samples from river/canal bank water sources and swab samples from door of transport vehicles and shop-counters were collected and analyzed for the load of pathogenic microorganism and possible disease potentials of these sources to prevent possible disease outbreak for the inhabitants. The results of study presents water samples collected from River/Canal bank contains the total aerobic bacterial counts ranged between 21×10⁵ to 16.6×10⁶ per ml, while on the door and seat handles of public transport vehicles and frequent public use shop counters the total bacterial counts ranged between 18×10⁴ to 17.5×10⁵ and 21×10⁴ to 11.3×10⁵, respectively. All of these were within the range of a high risk specified by WHO. In addition, There was a high correlation between water isolates with fecal isolates of different sources both in their culture characteristics as well as biochemical profiling which implies that the different sources might have been contaminated with mixed contaminants instigate from human as well as animal excreta. Forestalling of deterioration of water quality and pathogenic diseases transmission to humans via contaminated and improperly cleaned surfaces require community health superintendents, sanitary officers and Environmental Protection Board as well as private organizations to educate the population on personal and environmental hygiene.