Different bacterial strains were isolated from hospitalized patients with Urinary Tract Infection and microscopically and biochemically characterized as Escherichia coli, Klebsiella pneumoniae , Pseudomonas aeruginosa , Proteus mirabilis Streptococcus sp. , Staphylococcus saprophyticus and Alcaligenes faecalis . Klebsiella pneumoniae was the most common urine isolate and Staphylococcus saprophyticus was another common organism. The antimicrobial activity of vitamin C sources like cranberry, amla, lemon, roseberry and mango was determined against a total of forty eight isolated organisms by well plate method and sensitivity of antibiotics were also checked by Kirby-Baur disc diffusion method. On comparative analysis of vitamin C source food articles and antibiotic sensitivity against isolated organisms it was found that action of cranberry, amla, lemon, roseberry and mango have good effect as compare to different antibiotics. Among these sources cranberry was most effective against all urine isolates. Due to the emergence of drug resistant microorganisms, it is a need to search out more effective antimicrobial agents to cure the disease. Our studies suggest that high intake of vitamin C tends to increase the acidity of urine which is not well tolerated by the bacteria responsible for UTI.