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标题：Arsenic Induced Perturbations in Cholinergic System and Energy Metabolism in Young and Adult Rat Brain: Reversal Effect of Vitamin-E
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作者：C. BHUVANESWARI DEVI ; K. KIRAN KUMARI ; G. INDRAVATHI 等
期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2014
卷号：3
期号：10
页码：16840
DOI：10.15680/IJIRSET.2014.0310062
出版社：S&S Publications
摘要：Arsenic (As) occurs naturally as an element, ranks as the 20th most occurring trace element in the earth‟scrust. Arsenic is a known human carcinogen which acts via a genotoxic mechanism. Chronic exposure to inorganicarsenic compounds may lead to peripheral and central neurotoxicity. The aim of this study was to examine the impactof As exposure on cholinergic system and energy metabolism. In the present study the three months albino rats wereexposed to low dose (1.5 mg/kg body weight) and high dose (3 mg/kg body weight) of As through intraperitonialinjection daily for a period of 3 weeks. After the period of dosage, the As exposed animals were divided in to twogroups of which one group of both the doses were given Vitamin – E at a dose for a period of one week. The specificactivity of enzyme AChE and ACh content were estimated in synaptosomal fractions of cerebral cortex, hippocampusand cerebellum of control and all As exposed rats. The specific activity of enzymes Mg2+ ATPase and Na+K+ATPasewere determined in the mitochondrial fraction of cerebral cortex, hippocampus and cerebellum of control and all Asexposed rats. It was observed that AChE, Mg2+ ATPase and Na+K+ATPase activities were decreased and ACh contentwas increased in cerebral cortex, hippocampus and cerebellum of As-exposed (both low and high dose) rats whencompared to control rats. The effect of As was highly pronounced in high dose As exposed animals compared to thelow dose exposed rats. However, AChE, Mg2+ ATPase and Na+K+ATPase activities were increased and ACh contentwas decreased in the rats supplemented with Vitamin-E along with low dose and high dose of As. Among the threebrain regions the cerebral cortex was found to be more susceptible region towards As induced toxicity compared to the,hippocampus and cerebellum. This study demonstrates exposure to As provoked neuronal injury by inducingalterations in enzymes of cholinergic system and energy metabolism in dose dependent manner, where high doseexposure showed significant alterations compared to the low dose exposure. However, Vitamin-E treatment havepartial ameliorative effects on these disturbance caused by As toxicity.
关键词：Acetylcholinesterase (AChE); Acetylcholine (ACh); Mg2+ ATPase and Na+K+ATPase; Brain regions;Arsenic; Vitamin-E