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标题：Design and Characterization of DNA Aptamer for Breast Tumor Marker by an Advantageous Method
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作者：NANDITA P. AGNIHOTRI ; SHIKHA DUBEY ; MANGLA BHIDE 等
期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2014
卷号：3
期号：10
页码：16642
DOI：10.15680/IJIRSET.2014.0310030
出版社：S&S Publications
摘要：Aptamers are agents able to bind tightly and selectively to disease markers, offering great potential for applications in disease diagnosis and therapy. Functionally they are ssDNA or RNA oligonucleo tides which are highly specific and selective to their target along with highest affinity. We have selected DN A aptamer against breast tumor marker (CA 15-3) b y using a modified SELEX methodology. For the selection round CA 15-3 were immobilized on magnetic beads and to check the affinity of selected aptamers for their target fluorescent labeled aptamers were used. Aptamers were selected from an initial library containing 25 base long variable regions for their ability to bind to the CA 15-3. After 12 rounds of the selection and amplification we found 90% pool of DNA seq uences which are able to bind with CA 15-3. These aptamers were cloned, sequenced and labeled by fluorescent molecule. The binding affinity of these aptamers (K D value) was quantified by using FAM labeled aptamers. Our results aim to develop new diagnostic assays against breast tumor marker for the early diagnosis of breast cancer
关键词：Aptamer; SELEX; Breast Tumor; Magnetic beads; CA 15-3