文章基本信息

标题：Enhanced Production and Characterization of Commercially Important Thermostable Amylolytic Enzyme
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作者：Sarita Sharma ; Rachana Bhatt
期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2014
卷号：3
期号：7
页码：14525
出版社：S&S Publications
摘要：In the present study, different soil samples were analyzed to find a potent amylase producer. We found anatural soil isolate, named as Pseudomonas sp. B5 – a Gram negative short rod, which gave maximum amylaseproduction (12.79 μmole/ml/min) in production medium containing 3% soluble starch when incubated for 24 h at 150rpm. Further characterization of partially purified amylase proved it to be an enzyme having molecular weight of55,370 KDa with 1.8 μmole Km and 0.019 U/min Vmax. Enzyme showed pH optima of 5 with activity in wide range oftemperature ranging from 30 oC - 100 oC. This amylase was stable up to 35 minutes at 100 oC. Effect of severalinhibitors and metal ions on amylase activity was also checked. EDTA proved to be detrimental for the amylase whereas presence of metal ions namely Ca+2 and Fe+3 showed increase in the activity of the amylase.