出版社:Shahid Sadoughi University of Medical Sciences, yazd, Iran
摘要:Background: Unlike industrially production, Iranian traditional doogh are not pasteur-ized after production. Hence, possible contamination with different pathogenic bacteria may occur during production or post-production of traditional doogh. The aim of the present study was to monitor the behavior of Escherichia coli O157:H7 and Listeria monocytogenes in traditional Iranian doogh at different temperatures and time periods. Methods: Low acid and high acid doogh samples were inoculated with approximately 4 and 6 log cfu/ml of each pathogen separately and then incubated at 4 ºC and 25 ºC. At different time interval, samples were taken to enumerate E. coli O157:H7 and L. monocytogenes and determine titratable acidity. Results were analyzed by ANOVA test using SPSS software (v. 16.0). Results: Although the survival ability of E. coli O157:H7 in doogh samples was slightly higher than L. monocytogenes, both of them were detected in low acid doogh sample after 10-14 days at 4 ºC. However, they were not detected in low acid doogh samples for more than 2 days at 25 ºC. In contrast, in high acid doogh samples, the viability of E. coli O157:H7 and L. monocytogenes declined more quickly to undetectable level, both at 4 ºC and 25 ºC indicating less viability of these bacteria in high acid doogh samples compared to those of low acid ones (p