文章基本信息

标题：A NOVEL ALKALINE PHOSPHATASE ENZYME IN ACUTE LYMPHOBLASTIC LEUKEMIA PATIENTS’ SERUM
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作者：Mohammad Aberomand ; Amir Jalali ; Mohammad Pedram 等
期刊名称：Journal of Current Research in Science
印刷版ISSN：2322-5009
出版年度：2013
卷号：1
期号：6
页码：484-488
出版社：Islamic press
摘要：Alkaline phosphatase (ALP) is a non-specific member of a group of phosphomonoesterase enzymes generating an organic radical and inorganic phosphate due to catalyzing the hydrolysis of phosphate esters in an alkaline environment. The aim of the present study was to investigate the clinical usefulness of characterization of alkaline phosphatase isoenzymes, as metastases markers. The alkaline phosphatase activity of the Human Serum has been partially characterized in patients with lymphoblast leukemia's acute diseases and the ‘in vitro’ effect of heavy metals on enzymatic activity has been analyzed. The optimal pH was 10.2 for alkaline phosphatase activity. The effect of heavy metals is dependent on the serum of patients analyzed. Zn+2 showed the highest inhibition for normal serum enzyme activity but Mn+2 showed the highest inhibition for lymphoblast leukemia's acute cancer serum enzyme and the parameter Km For normal serum and cancer serum was 18 and 20 respectively. This is a different and unique isoenzyme which may use as a diagnostic marker in lymphoblast leukemia's acute patients due to increase in its activity, different km value, different pH optima, different effect of Zn+2 and Mg+2 comparing to another members of phosphomonoesterase group
关键词：Alkaline Phosphatases; pH; Metals; Human; Serum; lymphoblastic leukaemias acute