摘要:Allele frequency distribution in three Macrobrachium species (M. lamarrei, n=100; M. rosenbergii, n=100 and M. malcolmsonii, n=150) was counted on different esterase isozyme loci on 7.5% Polyacrylamide Gel Electrophoresis (PAGE) stained with α- and β- naphthyl acetates from the eye tissues. Altogether three (Est-11.00±0.02, Est-20.60±0.06 and Est-50.10±0.01), five (Est-10.99±0.03, Est-20.59±0.04, Est-30.46±0.02, Est-40.33±0.03 and Est-50.10±0.02) and four (Est-10.99±0.03, Est-20.66±0.09, Est-40.27±0.03 and Est-50.11±0.03) esterase bands were detected in the above three species in order. Est-5 band of M. lamarrei (94%) and that of M. rosenbergii (99%) and Est-4 band of M. malcolmsonii (89%) was found to be prominent in the sampled populations. Individuals, containing different number of bands, also varied within each species and each band showed a remarkable extent of polymorphism. Calculated data indicated that the population of M. lamarrei (χ2 = 31.12) and M. rosenbergii (χ2 = 24.63) was out of Hardy-Weinberg equilibrium (HWE) whereas M. malcolmsonii (χ2 = 7.13) was within equilibrium
关键词:Allele frequency; Esterase; Polymorphism; Macrobrachium species