Groundnut (Arachis hypogaea L.) is an annual legume which is also known as peanut, earthnut, monkeynut and ground bean. Among the major oil seed crops, groundnut has some specific advantages because of its use in many food products. The present study is planned to characterize different groundnut varieties, grown under similar conditions, for their chemical composition and nutritional assay. Fatty acids profile of groundnut will be determined through Gas Chromatography. The oleic acid (C18: 1) contents of groundnut varieties Bari-2000, P.W, 2KCG020, 2KCG017, 96CG005, Golden, No. 334, Chico, 01CG009 and 02CG005 were 43.6, 41.9, 36.4, 40.5, 38.0, 37.5, 35.8, 31.6, 30.4 and 32.9 g/100g respectively.