文章基本信息

标题：Production of Chicken Hyperimmune Hens' Egg for Immunological Purposes in Animals
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作者：Ismail. I. Abo-Ghanema ; Madiha S. Ibrahim ; Bahig. R. Nemettallah 等
期刊名称：Alexandria Journal of Veterinary Sciences
印刷版ISSN：1110-2047
电子版ISSN：1110-2047
出版年度：2016
卷号：49
期号：2
页码：133-140
DOI：10.5455/ajvs.224376
出版社：International Medical Journal Management and Indexing System
摘要：The present study was aimed to produce hyperimmune chicken egg against rabbit hemorrhagic disease virus (RHDV) as well as studying immunogenic characters of the extracted IgY using antigen-antibody reaction tests (agar gel immunodiffusion and ELISA). The study was carried out on ten white Hy-Line layers which received initial immunization of 300µl of RHDV vaccine subcutaneously followed by three booster doses at time intervals of 2, 5 and 8 weeks after initial immunization. Throughout the experiment (14 weeks), regular blood sampling was made every week with daily collection of egg till further purification and detection of the purity of the isolated IgY by SDS-PAGE. Also, determination of the specificity and titers of purified IgY in hens' sera and their purified egg yolk samples was carried out using agar gel immunodiffusion test and indirect ELISA. Results showed presence of considerable titers of isolated IgY against RHDV. Therefore, the current study recommends immunization of hens with RHDV for production of egg yolk antibodies against such pathogen.
关键词：Rabbit hemorrhagic disease virus; IgY; Indirect ELISA