文章基本信息

标题：Effective mRNA Inhibition in PANC-1 Cells in Vitro Mediated via an mPEG–SeSe–PEI Delivery System
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作者：Yuefeng Zhang ; Bin Yang ; Yajie Liu 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2016
卷号：39
期号：5
页码：680-688
DOI：10.1248/bpb.b15-00645
出版社：The Pharmaceutical Society of Japan
摘要：RNA interference (RNAi)-mediated gene therapy is a promising approach to cure various diseases. However, developing an effective, safe, specific RNAi delivery system remains a major challenge. In this study, a novel redox-responsive polyetherimide (PEI)-based nanovector, mPEG–SeSe–PEI, was developed and its efficacy evaluated. We prepared three mPEG–SeSe–PEI vector candidates for small interfering glyceraldehyde-3-phosphate dehydrogenase (siGADPH) and determined their physiochemical properties and transfection efficiency using flow cytometry and PEG_11.6–SeSe–PEI polymer. We investigated the silencing efficacy of GADPH mRNA expression in PANC-1 cells and observed that PEG_11.6–SeSe–PEI/siGADPH (N/P ratio=10) polyplexes possessed the appropriate size and zeta-potential and exhibited excellent in vitro gene silencing effects with the least cytotoxicity in PANC-1 cells. In conclusion, we present PEG_11.6–SeSe–PEI as a potential therapeutic gene delivery system for small interfering RNA (siRNA).