文章基本信息

标题：Lactobacillus strains
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作者：Sedláčková P. ; Horáčková Š. ; Shi T. 等
期刊名称：Czech Journal of Food Sciences
印刷版ISSN：1212-1800
电子版ISSN：1805-9317
出版年度：2015
卷号：33
期号：1
页码：13-18
DOI：10.17221/299/2014-CJFS
出版社：Czech Academy of Agricultural Sciences
摘要：Bile salt hydrolase (BSH) activity of intestinal bacteria (including lactobacilli) is one of the indirect ways of decreasing a cholesterol level in human body. Tested Lactobacillus strains were isolated from various sources (faeces of fully breast-fed infants, cow’s colostrum, cow’s raw milk cheeses, and cow’s raw milk) and identified by genotypic and phenotypic methods. All strains, including three commercial probiotic strains and six culture collection strains, were subsequently tested for their BSH activity via two methods – thin layer chromatography (TLC) and plate assay. Among all the 59 Lactobacillus strains, 15 strains were shown to be BSH positive by TLC method and only 8 of them by plate assay. Most of the BSH positive strains (9 strains) were isolated from faeces. Differences between BSH activities for sodium salts of cholyltaurine and cholylglycine were demonstrated. The TLC method was shown to be more sensitive compared to the plate assay for BSH activity determination.
关键词：Lactobacillus ; bile salt hydrolase activity