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  • 标题:Optimisation of ultrasonic-assisted protein extraction from brewer’s spent grain
  • 本地全文:下载
  • 作者:Tang D.-S. ; Tian Y.-J. ; He Y.-Z.
  • 期刊名称:Czech Journal of Food Sciences
  • 印刷版ISSN:1212-1800
  • 电子版ISSN:1805-9317
  • 出版年度:2010
  • 卷号:28
  • 期号:1
  • 页码:9-17
  • DOI:10.17221/178/2009-CJFS
  • 出版社:Czech Academy of Agricultural Sciences
  • 摘要:Response surface methodology was employed to optimise the ultrasonic-assisted extraction of protein from brewer’s spent grain. Three variables, namely the extraction time (min), ultrasonic power (W/100 ml of extractant), and solid-liquid ratio (g/100 ml) were investigated. Optimal conditions were determined and tri-dimensional response surfaces were plotted using mathematical models. The ANOVA analysis indicated that all the quantities determined, i.e. the extraction time, ultrasonic power, and solid-liquid ratio, had significant positive linear and negative quadratic effects on the protein yield. Optimum conditions for the extraction of protein were found to be: the extraction time of 81.4 min, ultrasonic power of 88.2 W/100 ml of extractant, and solid-liquid ratio of 2.0 g/100 ml. The optimal predicted protein yield obtained was 104.2 mg/g BSG while the experimental yield of protein was in agreement with the predicted value.
  • 关键词:ultrasonic-assisted extraction; protein; Brewer’s spent grain; response surface methodology download PDF Impact factor (Web of Science – Thomson Reuters) 2017: 0.868 5-Year Impact Factor: 1.107
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