文章基本信息

标题：Immobilized metal ion chromatographic (IMAC) determination of ovomucoid in hen’s egg white
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作者：Prošková A. ; Kučera J.
期刊名称：Czech Journal of Food Sciences
印刷版ISSN：1212-1800
电子版ISSN：1805-9317
出版年度：2002
卷号：20
期号：3
页码：95-97
DOI：10.17221/3517-CJFS
出版社：Czech Academy of Agricultural Sciences
摘要：An immobilized metal ion high-performance liquid chromatographic technique was developed for the determination of one of the egg allergens – ovomucoid – in egg white and food products containing egg white. The method was tested using standard samples as well as complete egg white and its reproducibility was determined. The possible application of this method is discussed. The method is based on the HPLC chromatography on the chelating column saturated with copper ions. The sample is applied to the column at pH 7.5 and eluted isocraticaly with a buffer containing 1.75 mM imidazole in the optimum case.
关键词：ovomucoid; determination; HPL.C; egg white; analytical method; IMAC; HPLC