文章基本信息

标题：Lactococcus lactis subsp. lactis LTM 32, a new bacteriocin-producing strain isolated from Vietnamese fermented milk
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作者：Do T.M. ; Plocková M. ; Chumchalová J. 等
期刊名称：Czech Journal of Food Sciences
印刷版ISSN：1212-1800
电子版ISSN：1805-9317
出版年度：2001
卷号：19
期号：5
页码：171-176
DOI：10.17221/6603-CJFS
出版社：Czech Academy of Agricultural Sciences
摘要：Screening for bacteriocin production of 60 strains of lactic acid bacteria (LAB) both isolated from various Vietnamese fermented food and obtained from the culture collection resulted in the detection of a bacteriocin-producing strain, Lactococcus lactis subsp. lactis LTM 32 isolated from Vietnamese fermented milk. This strain was identified and partly characterized. This bacteriocin inhibited not only closely relative strains of LAB but also strains of Listeria monocytogenes , Bacillus sp. including B. cereus , B. stearothermophilus , B. subtilis , B. pumilus and Staphylococcus aureus . It was heat stable at 121°C for 15 min, at 100°C for 120 min and stable during storage at –20°C and 4°C for 3 months. The bacteriocin was inactivated by some proteolytic enzymes, namely by proteinase K and pronase E in concentrations up to 0.5 mg/ml but not by α-amylase and pepsin. The optimal growth and bacteriocin production were achieved in M17L broth at 30°C and initial pH 6.8. The characteristics of bacteriocin-producing strain Lactococcus lactis subsp. lactis LTM 32 are of interest for food safety.
关键词：Lactococcus sp.; Listeria monocytogenes ; Staphylo coccus aureus ; Bacillus sp.; fermented milk; bacteriocin; nisin