文章基本信息

标题：Identification of a Lactic Acid Bacterial flora within the honey intestinal tract of Apis mellifera from different regions of Bulgaria
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作者：YAVOR RABADJIEV ; PETYA CHRISTOVA ; ILIA ILIEV 等
期刊名称：Journal of BioScience and Biotechnology
印刷版ISSN：1314-6238
电子版ISSN：1314-6246
出版年度：2015
页码：215-219
出版社：Plovdiv University Press
摘要：In the present study, we analyzed LAB microflora in the intestinal tract of bees Apis melifera on the territory of Bulgaria. Sixteen isolates were collected from the honey stomach of the honeybee Apis mellifera. Poor growth was recorded when strains were incubated anaerobically in the presence of D-glucose as sole carbon source. All of isolated strain showed fructophilic characteristics fermenting fructose faster than glucose. The isolates were Gram-stained and tested for catalase reaction. The 16S rRNA genes from extracted DNA of bacterial colonies were amplified with polymerase chain reaction using universal primers 27F and 1492R and were sequenced. The 10 isolated strains yielded five distinct 16S sequences of Lactobacillus plantarum, L. pentosus, L. iwatensis, L. kunkeei, Weissella Confusa. DNA of sequenced strains were amplified with specific primers in order to confirm the genus of the samples LBMA-1 (CTCAAAACTAAACAAAGTTC) and R16-1 (CTTGTACACACCGCCCGTCA). Carbohydrate fermentation reactions were recorded using API 50CH. For detection of enzymes activities of strains was used API ZYM. These strains can be good candidates for potential application as probiotics in honeybees and also as natural food preservatives, which, in turn, may be useful in the food industry
关键词：fructofilic lactic acid bacteria; Apis melifera; Lactobacillus kunkeei; Weissella confusa