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标题：ANALIZA HIDROLIZEI VICILINEI ŞI FAZEOLINEI DE CĂTRE PAPAINĂ LA pH ACID
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作者：Tatiana STEPURINA ; Natalia GAŞINA ; Diana MORARI 等
期刊名称：Studia Universitatis Moldaviae: Stiinte Exacte si Economice
印刷版ISSN：1857-2073
电子版ISSN：2345-1033
出版年度：2009
期号：6(26)
页码：121-127
出版社：Moldova State University
摘要：The vicilin and phaseolin proteolysis by papain at acidic pH has been studied in vitro. It has been found that vetch vicilinis practically completely degradated at pH 4.6. The phaseolin hydrolysis differs from that of vicilin, phaseolin beingonly modifi ed by papain. This modifi cation results in the formation of fragments that correspond to half subunits of nativephaseolin that are resistant to further action of papain. These fragments remain associated in molecule’s quaternary structureproducing phaseolin-Pap. The phaseolin modifi ed at pH 4.6 becomes insoluble, while at pH 5.6 it is soluble. Thesefi ndings show that while the action of papain on vicilin is similar to the action of endogenouse papin-like proteinases itsaction on phaseolin is slightly different