文章基本信息

标题：EXRACTIBILITY OF HIV-1 POL-INTEGRASE FROM PLASMA SPOTS AFTER 4 WEEKS OF STORAGE AT ROOM TEMPERATURE.
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作者：Felix Dambula ; Victor Makene
期刊名称：African Journal of Science and Research
印刷版ISSN：2306-5877
出版年度：2016
卷号：5
期号：2
页码：04-06
出版社：Research, Science and Technology publishers
摘要：Although least studied variant of dried blood spot (DBS), dried plasma spots (DPS) have been recommended as the ideal sample not only for HIV-1 RNA quantification, but also drug resistance screening and genotyping. Different Assays have been invented that target different genomic regions of the virus including the pol-integrase region targeted by the Abbott m2000rt assay. However, since in many third world countries before testing is done there’s a lot of dried fluid spot sample storage and transportation from peripheral clinics to the testing site. This study, which was part of a larger study, tested whether DPS prepared at room temperature (~25oC) can still provide extractible HIV-1 RNA on Abbott m2000rt assay after 1 month of storage at room temperature. Tested against DBS as control, we found that the pol-integrase region of HIV-1 on DPS is still viable and that all together quantifiable on Abbott m2000rt Assay
关键词：Plasma Spot; Pol-Intergrase Viability; Abbott M2000rt; Mzuzu