文章基本信息

标题：Study the influence of temperature on activity of fructosyltransferases by strain Leuconostoc mesenteroides Lm 17
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作者：TONKA VASILEVA ; VESELIN BIVOLARSKI
期刊名称：Journal of BioScience and Biotechnology
印刷版ISSN：1314-6238
电子版ISSN：1314-6246
出版年度：2012
页码：15-19
出版社：Plovdiv University Press
摘要：In the present work we studied the enzyme reaction of extracellular and cell-associated fructosyltransferase from Leuconostoc mesenteroides Lm 17 at different temperatures in order to estimate their influence on the enzyme activity. We measured the enzyme activity at a temperature range from 30°C to 45°C in the presence of 10% raffinose as a specific substrate for fructosyltransferases (FTFs). The highest extracellular and cell-associated FTF activities were detected at different temperatures – 0,29 U/mg at 30°C and 0.72 U/mg at 40°C, respectively. We did not detect extracellular FTF activity at 35°C. At 45°C a strain Lm 17 retained only 10% of the maximum enzyme activity detected at 40°C. We found that the studied strain produces mainly cell-associated FTFs which were 71% of the total FTF activity and are more stable at higher temperature. The received results were compared to detected extracellular and cell-associated FTF activities for the referent strain NRRL B-1149.In order to compare the type of produced FTFs from Lm 17 with referent strains Leuconostoc mesenteroides NRRL B-1149, NRRL B-512F and ATCC 8293, which are known as FTF producers, we performed in situ analysis after SDS-PAGE of extracellular and cell-associated enzyme fractions. The in situ analysis after Periodic Acid - Schiff′s staining procedure showed that all strains produce comparable FTFs with molecular weights corresponding to 120 kDa and 86 kDa
关键词：fructosyltransferases; Leuconostoc mesenteroides; raffinose