文章基本信息

标题：Qualitative and quantitative determination of protopine in Fumaria spp . by TLC-densitometry method
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作者：RADKA VRANCHEVA ; IVAN IVANOV ; ANDREY MARCHEV 等
期刊名称：Journal of BioScience and Biotechnology
印刷版ISSN：1314-6238
电子版ISSN：1314-6246
出版年度：2012
卷号：1
期号：3
页码：255-259
出版社：Plovdiv University Press
摘要：A rapid and accurate TLC-densitometry method for qualitative and quantitative determination of protopine has been developed. The best separation was achieved using a mobile phase chloroform – ethyl acetate – methanol – ammonium hydroxide (80:80:40:0.05, v/v/v/v). The results obtained by this method (CV% 3.4) corresponded well with those obtained by using a HPLC method. The reliability of the proposed method was proved through reproducibility test with alkaloid extracts from Fumaria spp
关键词：TLC; protopine; HPLC; Fumaria officinalis L.; Fumaria rostellata Knaf.