文章基本信息

标题：Effects of the insecticide "Actara 25 WG" on the glyconeogenesis in the liver of common carp ( Cyprinus carpio L.)
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作者：STELA STOYANOVA ; ELENKA GEORGIEVA ; ILIANA VELCHEVA 等
期刊名称：Journal of BioScience and Biotechnology
印刷版ISSN：1314-6238
电子版ISSN：1314-6246
出版年度：2012
卷号：1
期号：3
页码：249-254
出版社：Plovdiv University Press
摘要：The main goal of the present work is to study the effects of the new neonicotinoid insecticide „Actara 25 WG“ on the intensity of expression of glycogen in the liver of common carp (Cyprinus carpio L.) by using PAS-reaction on cryosections. Common carp is an economically important fish species, which is widely used as a bioindicator for the health of freshwater basins since it could also survive at very contaminated sites. We have used 6.6 mg/L, 10 mg/L and 20 mg/L of the test chemical under laboratory conditions. The results demonstrated that the intensity of staining of the PAS-reaction is directly proportional to the increasing concentration of the insecticide. In addition, this indicates that the amount of glycogen in hepatocytes also increased. Conglomerates of accumulated glycogen in certain hepatocytes were found at the highest concentration of the insecticide. Therefore, we consider that under the influence of „Actara 25 WG“ the process of glyconeogenesis in the liver of the studied fish accelerates
关键词：histochemistry; PAS-reaction; liver; common carp (Cyprinus carpio L.); pesticide