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标题：Influence of extracts from Mentha aquatica L. and Mentha longifolia L. on the activity of glycosyltransferases from Leuconostoc mesenteroides strains
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作者：PETKO BOZOV ; RUMEN MLADENOV ; IVANKA DIMITROVA 等
期刊名称：Journal of BioScience and Biotechnology
印刷版ISSN：1314-6238
电子版ISSN：1314-6246
出版年度：2014
页码：61-66
出版社：Plovdiv University Press
摘要：A study of the influence of different extracts from Mentha aquatica L. and Mentha longifolia L. on the activity of glycosyltransferase complex from Leuconstoc nesenteroides URE 13 and dextransucrase from Leuconostoc mesenteroides Lm 28 was carried out. The extracts from two plant species showed an inhibitory effect on studied glycosyltransferases at minimal concentration of the total extract 1.87 mg/ml in the reaction mixtures. At this concentration the enzyme complex from URE 13 strain lost about 18% (M. longifolia L.extract) and 12% (M. aquatica L.extract). For dextransucrase from Lm 28 strain a higher inhibition varying from 22% (M. longifolia extract) to 30% (M. aquatic extract) was detected. When the concentration of the extracts was raised to 9.35 mg/ml, the inhibitory effect on the enzyme complex from URE 13 and dextransucrase from Lm 28 strain increase. At this concentration of the extracts a higher percent of inhibition was detected for the enzyme from Lm 28 strain – 100% for the extract from both M. longifolia L and 98% for this from M. aquatica L. For the enzyme from L. mesenteroides URE 13 strain, at concentration 9.35 mg/ml was determined 97% inhibition for both plant extracts. The inhibitory effect of both extracts on the studied enzymes was compared with equivalent concentrations of essential oil from M. piperita L. where an inhibitory effect ranging from 63% (URE 13 enzyme complex) to 76% (Lm 28 dextransucrase) was observed. The effect of the extracts from both Mentha sp. on transferase to hydrolase activity ratio was also studied and showed predominance of transferase activity with more than 90% at 24 h from the start of the reactions
关键词：glycosyltransferases; Leuconostoc mesenteroides; Mentha aquatica L.; Mentha longifolia L.