文章基本信息

标题：Cloning and expression of gene encoding P23 protein from Cryptosporidium parvum
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作者：DINH THI BICH LAN ; TRAN THUY LAN ; LE QUOC VIET 等
期刊名称：Journal of BioScience and Biotechnology
印刷版ISSN：1314-6238
电子版ISSN：1314-6246
出版年度：2014
卷号：3
期号：3
页码：189-193
出版社：Plovdiv University Press
摘要：We cloned the cp23 gene coding P23 (glyco)protein from Cryptosporidium parvum isolated from Thua Thien Hue province, Vietnam. The coding region of cp23 gene from C. parvum is 99% similar with cp23 gene deposited in NCBI (accession number: U34390). SDS-PAGE and Western blot analysis showed that the cp23 gene in E. coli BL21 StarTM (DE3) produced polypeptides with molecular weights of approximately 37, 40 and 49 kDa. These molecules may be non-glycosylated or glycosylated P23 fusion polypeptides. Recombinant P23 protein purified by GST (glutathione S-transferase) affinity chromatography can be used as an antigen for C. parvum antibody production as well as to develop diagnostic kit for C. parvum.
关键词：cp23 gene; Cryptosporidium parvum; P23 protein