文章基本信息

标题：Reference Genes: Essential Criteria for Assessment of the Real-Time PCR Based Virus Detection in Plants Virology
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作者：Rongfei Lu ; Zhiyang Liu ; Peng Fang 等
期刊名称：British Journal of Virology
电子版ISSN：2055-6128
出版年度：2016
卷号：3
期号：1
页码：6-10
DOI：10.17582/journal.bjv/2016.3.1.6.10
出版社：Smith and Franklin Academic Publishing Coporation
摘要：Real-time quantitative PCR (RT-qPCR). plays an important role in the current field of virus detection and disease dynamics. The validity and the reliability of generated data can be enhanced significantly appropriate measures. As a standard for the relative expression of target genes, the selection of reference genes is crucial. This review describes the history of the RT-qPCR technology,. emphasizes. the importance of reference genes and enumerates several algorithms to screen reference genes to normalize the RT-qPCR data. Additionally, several possible improvements in the selection of reference genes are discussed.
关键词：RT-qPCR; Reference gene selection; Multiple; Algorithms