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标题：Experimental approach for bacteriophage susceptibility testing of planktonic and sessile bacterial populations – Study protocol
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作者：Alina Cristina Neguț ; Oana Săndulescu ; Marcela Popa 等
期刊名称：Germs
印刷版ISSN：2248-2997
出版年度：2014
卷号：4
期号：4
页码：92-96
DOI：10.11599/germs.2014.1062
出版社：European HIV/AIDS and Infectious Diseases Academy
摘要：Introduction Antimicrobial resistance is a growing threat for all clinical branches. This phenomenon poses important challenges in controlling infectious diseases. However, multidrug resistance is not the only issue, as bacteria that are otherwise susceptible to common antibiotics express other patterns for evading antibiotherapy,for example they can aggregate within a self-produced matrix to form biofilm. Methods We intend to perform a prospective laboratory study of the germs isolated from different samples collected from patients admitted with infectious pathology in reference hospitals in Romania. We will perform antibiotic resistance testing as well as phage testing, both on solid and liquid growth medium, for Staphylococcus spp., Enterococcusspp., and Pseudomonas spp. We intend to collect data for 150 patients with different infections with these identified pathogens.Phage susceptibility testing will be performed using 5 types of strain-specific bacteriophage mixtures: PYO, INTESTI, STAPHYLOCOCCAL (Eliava BioPreparations, Tbilisi, Georgia), PHAGYO, PHAGESTI (JSC "Biochimpharm", Tbilisi, Georgia). For phage-susceptible strains, we will evaluate biofilm formation in the presence of phages, as well as phage effect on already formed biofilm.Expected results Through this study, we intend to provide the first set of results on bacteriophage-susceptibility of bacteria isolated from patients with hard to treat infections, from reference hospitals in Romania. By evaluating a large number of bacterial strains we aim to predict and project biofilm kinetics, while adding binary phage dilutions at key timepoints during biofilm formation.
关键词：Bacteriophages; planktonic bacteria; biofilm; lysis; spectrophotometry