The subject of this paper is the carved wooden pulpits of the Church of Our Lady of Pena in Lisbon, Portugal. The fact that these objects were not gilded (as the rest of the carved wooden decoration in the church) raised the reinforced interest in their analysis. Besides the historical and stylistic approach, the text covers aspects of the materiality of the pulpits, including the wooden support and a grayish white layer. Analytical techniques such as optical microscopy complemented with the use of dyes to map the possible presence of binder materials constituting the white layer applied on the wooden support, scanning electron microscopy with energy dispersive X-ray spectrometry, X-ray diffraction, X-ray fluorescence spectrometry and micro-Fourier transformed infrared spectrometry were used to identify the components of paint materials.