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标题：A COMPARISON BETWEEN THE EFFECTS OF ALBENDAZOLE AND MEBEN DAZOLE ON THE ENZYMATIC ACTIVITY OF EXCRETORY / SECRETORY PROD-UCTS OF ECHINOCOCCUS GRANULOSUS PROTOSCOLECES IN VITRO
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作者：ADNANI SADATI SEYED JAFAR ; FARAHNAK ALI ; MOLAEI RAD MOHAMMAD BAGHER 等
期刊名称：Iranian Journal of Public Health
印刷版ISSN：2251-6085
电子版ISSN：2251-6093
出版年度：2016
卷号：45
期号：2
页码：223-229
语种：English
出版社：Tehran University of Medical Sciences
摘要：Background: Hydatid cysts are formed in human body can be treated clinically by surgery or drugs such as albenda-zole (ABZ) and mebendazole (MBZ). The purpose of this study was comparing the effects of ABZ and MBZ on glu-tathione-S-transferase, alkaline phosphatase and protease enzymes activities in protoscoleces of hydatid cyst.
Methods: The culture supernatants containing the parasite Excretory / Secretory (E/S) products were collected every 12 h for 72 h. The E/S products of treated samples with 1 m g/ml ABZ and MBZ and the control one were collected and after centrifugation then protein concentrations were measured according to Bradford method. GST, ALP and protease activities of E/S products were assessed photometrically.
Results: The mean of GST specific activity level in treated protoscoleces with ABZ and MBZ and in control group were obtained 69.44, 132.83 and 225.47U/mg/protein/ml respectively. The mean ALP activity level in treated protos-coleces with ABZ and MBZ and in control group were detected 19.22, 22.27 and 27.85 U/mg/protein/ml respective-ly. The protease activity level in treated protoscoleces with ABZ and MBZ were not detected. While the mean of pro-tease activity level in control group was 7.61U/mg/proteins. Statistical analysis showed the significant difference be-tween protein concentrations, the specific activities of GST, ALP and protease enzymes in treated protoscoleces in comparison with control group (P<0.05). Also, the significant difference were seen between specific activities of GST and ALP enzymes in treated protoscoleces with ABZ in comparison with treated group with MBZ (P<0.05).
Conclusion: ABZ is more effective on the enzymes activities (GST and ALP) as compared with MBZ.
关键词：HYDATID CYST PROTOSCOLECES; ALBENDAZOLE; MEBANDAZOLE; PROTEASE; GLUTATHIONE S-TRANSFERASE; ALKALINE PHOSPHATASE