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标题：Prevalence and species identification of Sarcocystis in raw hamburgers distributed in Yazd, Iran using PCR-RFLP
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作者：B. Hajimohammadi ; A. Dehghani ; M. Moghadam Ahmadi 等
期刊名称：Journal of Food Quality and Hazards Control
印刷版ISSN：2345-685X
电子版ISSN：2345-6825
出版年度：2014
卷号：1
期号：1
页码：6
语种：English
出版社：Shahid Sadoughi University of Medical Sciences, yazd, Iran
摘要：Introduction: Hamburger is a popular type of fast foods consumed all over the world. Sarcocystis spp. is a zoonotic parasitic pathogen which endangers safety of meat and meat products. The present study describes the prevalence rate of Sarcocystis spp. in hamburgers in Yazd, Iran using PCR-RFLP. Materials and methods: Raw hamburger samples (100 traditional and 90 industrial) from central region of Iran, Yazd were randomly selected. The genomic DNA was extracted using salting out method. Detection and identification of Sarcocystis isolates was performed using PCR-RFLP. Results: The results showed that 77.9% of all tested hamburger samples were infected with Sarcocystis spp. The infection rate in the traditional hamburger (87%) was significantly (p 0.05). Conclusion: Having zoonotic significance, considerable infestation rate of S. hominis seems to be high in hamburgers of this region of Iran. To the best of our knowledge, this is the first of its own kind carried out in hamburger samples in Iran.