文章基本信息

标题：Cloning and expression of Ketol-acid reductoisomerase (KARI) from Staphylococcus aureus
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作者：Y. Sandhya Rani ; P. Vijayalakshmi
期刊名称：Asian Journal of Biological and Life Sciences
印刷版ISSN：2278-747X
电子版ISSN：2278-5957
出版年度：2012
卷号：1
页码：79-82
出版社：Pbspublishing
摘要：Ketol-acid reductoisomerase (EC 1.1.1.86) catalyzes the conversion of 2-aceto-2-hydroxyacids to 2-keto-3hydroxyacids and their subsequent reduction by NADPH to 2, 3-dihydroxyacids. The ilvC gene encoding the Staphylococcus aureus enzyme was cloned and expressed in E. coli carrying pRSET vector. The enzyme expression was tested based on the blue white screening of the transformed colonies. The work was carried out to enable the over expression of KARI and thereby can be used as a drug target