文章基本信息

标题：Direct Detection of Bacillus ceruse Enterotoxin Gene by Multiplex PCR Kit in Dairy Products
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作者：Samira Amiri ; Ali Mohamadi Sani
期刊名称：Asian Journal of Biological and Life Sciences
印刷版ISSN：2278-747X
电子版ISSN：2278-5957
出版年度：2015
卷号：4
页码：194-197
出版社：Pbspublishing
摘要：Due to the heat resistance of Bacillus cereus, its potential pathogenic character, the capability to grow in milk and reported diseases upon consumption of dairy products, the organism should be considered as hazardous in pasteurized milk. In this study hblA, hblC and nheB genes was investigated. Enterotoxin producing ability of 25 raw milk and 5 pasteurization milk samples was judged by Multiplex PCR reactions. Results showed that hblA, hblC and nheB genes were found to be in 66.36, 23.33 and 66.56 percent of milk samples, respectively. Also, 16.66 % and 13.33 % of samples were contained hblA+hblC and all three genes, respectively. Our findings indicated that hblA, hblC and nheB genes specific Multiplex PCR can be employed for differentiation of enterotoxigenic B. cereus isolates