文章基本信息

标题：Antioxidant activity and fructan content in root extracts from elecampane ( Inula helenium L.)
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作者：NADEZHDA PETKOVA ; RADKA VRANCHEVA ; DASHA MIHAYLOVA 等
期刊名称：Journal of BioScience and Biotechnology
印刷版ISSN：1314-6238
电子版ISSN：1314-6246
出版年度：2015
卷号：4
期号：1
页码：101-107
出版社：Plovdiv University Press
摘要：The purpose of the present study was to investigate the antioxidant activity and fructans content in ethanol and water extracts of roots of elecampane ( Inula helenium L.), a Bulgarian medicinal plant. The extraction procedure included 95% (v/v) ethanol extraction and subsequent water treatment. The antioxidant activity was evaluated by several reliable methods such as DPPH-, ABTS-, FRAP- and CUPRAC-assays, as well as the total phenolic content. In addition, the total fructans and sugar content were determinated by spectrophotomeric, TLC and HPLC-RID methods. The level of fructans in ethanol extracts was 14.1 g / 100 g dry weight, as nystose and 1-kestose were only 0.3 g / 100 g dry weight, and 0.5 g / 100g dry weight, respectively. The absence of fructooligosacharides and sugars in water extracts after the ethanol pretreatment was established. Inulin content was evaluated to be 32 g / 100 g dry weight. The metabolites profile of roots revealed their potential application as radical scavengers due to the presence of polyphenols. Therefore, the root extracts of elecampane could be assumed as a rich source of biologically active substance, in particular dietary fiber with potential prebiotic effect, due to the presence of polysaccharide inulin and fructooligosacharides
关键词：Inula helenium L.; inulin; total phenolic content; antioxidant activity