文章基本信息

标题：To Study the Effect of Activated Charcoal, Ascorbic Acid and Light Duration on InVitro Micropropagation of Aloe vera L
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作者：Priyanka Das ; Alok Kumar Srivastav
期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2015
卷号：4
期号：5
页码：3131
DOI：10.15680/IJIRSET.2015.0405091
出版社：S&S Publications
摘要：The research was conducted to determine the effect of ascorbic acid (50 mgl-1, 100 mgl-1, 200 mgl-1)and activated charcoal (0.5 gl-1, 1 gl-1, 2 gl-1) independently with different light duration (darkness for 4 weeks, and16 hours light for 4 weeks followed by 2 weeks in 16 hours light) on shoot regeneration. Explants of Aloe vera wereplanted on MS basal media supplemented with 1.6 mgl-1 IAA, 4.0 mgl-1 BAP and cultured for 4 weeks. After 4 weeks,degree of explant browning was evaluated. Explants were then cut vertically into two pieces and planted on shootregeneration media. After 4 weeks more number of shoot developed on shoot regeneration medium, i.e. MS mediumsupplemented with 1.6 mg/l IAA + 4.0 mg/l BAP + 100 mg/l ascorbic acid + 0.5g/l activated charcoal. Browning of explantswas minimized in this medium and elongation of micro-shoots and the growth of the plantlets were also better.Further elongation and rooting of micro-shoots were obtained when sub-cultured on to MS basal medium containing0.5g/l activated charcoal and 100% of the survival of rooted plantlets was observed after acclimatization. Therefore, theabove protocol could be effectively used in rapid mi- cropropagation of elite plants of Aloe vera.
关键词：Aloe vera; Explant browning; Micropropagation; Growth hormones; Light duration; Culture media;Phenolic compounds