期刊名称:International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN:2347-6710
电子版ISSN:2319-8753
出版年度:2015
卷号:4
期号:1
页码:18763
DOI:10.15680/IJIRSET.2015.0401057
出版社:S&S Publications
摘要:In vitro flowering and an efficient micropropagation protocol was developed for the annual medicinalherb Physalis minima (Solanaceae) by the in vitro culture of nodal segments of mature plant. Murashige and Skoog’s(MS)medium, 3% sucrose and0.8% agar supplemented with 4.0mg/l BAP and 0.25mg/l 2,4 - D and1.0mg/l KIN and0.25mg/l NAA were found to develop fully functional flowers. MS medium supplemented with 2.0mg/l BAP and0.25mg/l IAA induced an average of three shoots per explants. Full strength MS medium with 2.0mg/l IBA exhibitedthe best in in vitro rooting. Seventy percent of the rooted shoots survived when transferred to green house and next tothe field condition.