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标题：Revealing the kinetics of Leishmania chagasi infection in the male genital system of hamsters
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作者：Amanda P. N. Quintal ; Bruna C. Borges ; Paula C. Brígido 等
期刊名称：Infectious Diseases of Poverty
印刷版ISSN：2049-9957
出版年度：2016
卷号：5
期号：1
页码：29
DOI：10.1186/s40249-016-0122-0
语种：English
出版社：BioMed Central
摘要：Leishmaniasis causes alterations and lesions in the genital system, which leads to azoospermia and testicular atrophy in animals during the chronic phase of the infection. The aim of this study was to reveal the kinetics of Leishmania chagasi infection in the genital system of male golden hamsters (Mesocricetus auratus). Animals were intraperitoneally inoculated with amastigotes from L. chagasi. At different time points animals were euthanized and genital organs processed for histo-pathological, qPCR, cytokines and testosterone detection assays. Our results showed a high parasite load in testis, followed by an increase of pro-inflammatory cytokines IL1-β, TNF-α and IFN-γ, and testosterone. Subsequently, IL-4 expression was upregulated and basal parasite persistence in testis was observed using the experimental approach. Extracellular amastigotes migrated to the epididymis posing as a potential major factor of parasite persistence and venereal transmission of L. chagasi infection in hamsters.
关键词：Leishmania chagasi ; Extracellular amastigotes ; Testis ; Epididymis ; Immune response ; Testosterone