文章基本信息

标题：Consuming algal products: trophic interactions of bacteria and a diatom species determined by RNA stable isotope probing
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作者：Melanie Sapp ; Gunnar Gerdts ; Marco Wellinger 等
期刊名称：Helgoland Marine Research
印刷版ISSN：1438-3888
出版年度：2008
卷号：62
期号：3
页码：283-287
DOI：10.1007/s10152-008-0110-2
出版社：BioMed Central
摘要：Heterotrophic marine bacteria utilise a wide range of carbon sources. Recently, techniques were developed to link bacterial identity and physiological capacity of microorganisms within natural communities. One of these methods is stable isotope probing (SIP) which allows an identification of active microorganisms using particular growth substrates. In this study, we present the first attempt to analyse bacterial communities associated with microalgae by rRNA-SIP. This approach was used to analyse bacterial populations consuming algal products of Thalassiosira rotula by applying SIP followed by reverse transcription of 16S rRNA and denaturing gradient gel electrophoresis. Generally, our results indicate that bacteria which consume algal products can be detected by isotope arrays coupled with fingerprinting methods.
关键词：Bacteria;phytoplankton interaction;Stable isotope probing;RT-PCR;DGGE