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标题：Culturable heterotrophic bacteria from the marine sponge Dendrilla nigra : isolation and phylogenetic diversity of actinobacteria
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作者：Joseph Selvin ; R. Gandhimathi ; G. Seghal Kiran 等
期刊名称：Helgoland Marine Research
印刷版ISSN：1438-3888
出版年度：2009
卷号：63
期号：3
页码：239-247
DOI：10.1007/s10152-009-0153-z
出版社：BioMed Central
摘要：Culturable heterotrophic bacterial composition of marine sponge Dendrilla nigra was analysed using different enrichments. Five media compositions including without enrichment (control), enriched with sponge extract, with growth regulator (antibiotics), with autoinducers, and complete enrichment containing sponge extract, antibiotics, and autoinducers were developed. DNA hybridization assay was performed to explore host specific bacteria and ecotypes of culturable sponge-associated bacteria. Enrichment with selective inducers (AHLs and sponge extract) and regulators (antibiotics) considerably enhanced the cultivation potential of sponge-associated bacteria. It was found that Marinobacter (MSI032), Micromonospora (MSI033), Streptomyces (MSI051), and Pseudomonas (MSI057) were sponge-associated obligate symbionts. The present findings envisaged that “Micromonospora–Saccharomonospora–Streptomyces” group was the major culturable actinobacteria in the marine sponge D. nigra. The DNA hybridization assay was a reliable method for the analysis of culturable bacterial community in marine sponges. Based on the culturable community structure, the sponge-associated bacteria can be grouped (ecotypes) as general symbionts, specific symbionts, habitat flora, and antagonists.
关键词：Sponge bacteria;Culturable bacteria;Bacterial endosymbionts;Dendrilla nigra;Actinobacteria