文章基本信息

标题：Measuring some flounder ( Platichthys flesus L.) innate immune responses to be incorporated in effect biomonitoring concepts
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作者：Andreas Skouras ; Dieter Steinhagen
期刊名称：Helgoland Marine Research
印刷版ISSN：1438-3888
出版年度：2003
卷号：57
期号：3
页码：199-205
DOI：10.1007/s10152-003-0140-8
出版社：BioMed Central
摘要：For an implementation of innate immune responses of flounder (Platichthys flesus) in an integrated biological effect monitoring concept, leucocytes were isolated from peripheral blood, head kidney and spleen, and analysed for their capacity to mount a respiratory burst response upon phorbol ester stimulation. Responding cells were identified by reduced nitro-blue-tetrazolium salt deposits and by dihydro-rhodamine fluorescence in light microscope and flow cytometric analysis. Responding cells were found in head kidney derived cell suspensions rather than in peripheral blood or spleen. Parallel cytometric and microscopic analysis indicated that responding cells had a granulocyte or monocyte morphology, were alpha-naphtyl-esterase or myeloperoxidase positive and in flow cytometry exhibited a characteristic forward and side scatter (FSC/SSC) pattern. These cells represented 30–40% of head kidney derived cell suspensions and only 4–5 % of peripheral blood and spleen. In order to reduce sampling effort in field studies, leucocyte cell suspensions derived from flounder head kidney could be used in respiratory burst assays without further enrichment protocols. This paper combines, for the first time, conventional and cytometric analysis of phagocytes derived from flounder peripheral blood and head kidney.
关键词：Flounder leucocyte;Lysozyme;Respiratory burst