文章基本信息

标题：The use of genetic bioassays to evaluate the environmental quality in a region under the influence of urban waste in Guaíba lake basin (Brazil)
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作者：Izabel Vianna Villela ; Juliana da Silva ; Iuri Marques de Oliveira 等
期刊名称：Journal of Environmental Science and Water Resources
印刷版ISSN：2277-0704
出版年度：2013
卷号：2
期号：5
页码：138-149
出版社：Wudpecker Journals
摘要：The mussel species Limnoperna fortunei was chosen as biomonitoring organism in Guaíba Lake Basin, based on population d ata, distribution, and sensitivity. Previous in vitro and in vivo studies studies with Single Cell Gel Assay (SCGA) and Micronuclei test (M N ) on this freshwater mussel showed that it is successful in biomonitoring studies, especially in urban pollution mon itoring. T his study evaluated two sampling sites in the Guaíba Lake (Guaíb a PC e Guaíba BR) , near urban waste discharges, and a control site (Itapu.) insight a preserved area, using L. fortunei individuals . Comparing to the control site, t h e Guaíba BR samp le induced DNA damage in haemocytes of mussels sampled both in situ and exposed to laboratory conditions, whereas MN only in in situ collected mussels. This sample also presented the onl y surface water mutagenic result by Salmonella /microsome assay with TA 98 in the presence of metabolic activation. Guaíba PC samples increased MN frequency in situ and in laboratory conditions comparing to the Itapu. results . Metal influence seems to be less important than organic influence in genotoxic induction. These resul ts confirm the strong urban influence in this region, showing that biomonitoring is a powerful tool to detect this kind of contamination in water bodies.
关键词：Limnoperna fortunei; urban contamination; comet assay; micronucleus test; Ames test; Guaíba basin