文章基本信息

标题：Cattle urine increases lipid content in Chlorella pyrenoidosa: A low cost medium for bioenergy application
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作者：N. Sharma ; M. P. Rai
期刊名称：Iranica Journal of Energy and Environment (IJEE)
印刷版ISSN：2079-2115
电子版ISSN：2079-2123
出版年度：2015
期号：4
页码：334-339
语种：English
出版社：Noshirvani University of TEchnology
摘要：Media requirement for microalgae cultivation adds most to the cost of biodiesel production at commercial scale. The present work aims to study the growth of green algae Chlorella pyrenoidosa under fogg`s medium and modified fogg`s medium by replacing KNO3 with urea at different concentrations 0.15, 0.20 and 0.25% (w/v). To reduce the cost of urea, cow urine (CU) was utilized to grow the Chlorella sp. in different volume fractions as 5, 7.5, 10, and 12.5 % (v/v). Biomass production in 7.5% cow urine was achieved 1.93 g l -1 that is almost double in comparison to normal Fogg’s medium (0.82 g l -1 ). Cellular biochemical components such as lipid, protein and carbohydrate are determined quantitatively. The lipid content is found to be 32.7 % in 10 % of cow urine and 22.7 % in 7.5 % cow urine that is much higher than the Chlorella sp. grown under Fogg`s media (7%). The protein content is enhanced to 50.17% and carbohydrate reduced by half in comparison to normal fogg’s medium cells. The extracted lipid is converted into fatty acid methyl esters (FAME) and characterized by GC-MS. The FAME produced from cow urine grown cells showed suitable composition to prove its application as biofuel.