文章基本信息

标题：EFFECT OF GENISTEIN AND L-CARNITINE AND THEIR COMBINATION ON GENE EXPRESSION OF HEPATOCYTE HMG-COA REDUCTASE AND LDL RECEPTOR IN EXPERIMENTAL NEPHROTIC SYNDROME
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作者：YOUSEFINEJAD ABBAS ; SIASSI FEREYDOON ; MIRSHAFIEY ABBAS 等
期刊名称：Iranian Journal of Public Health
印刷版ISSN：2251-6085
电子版ISSN：2251-6093
出版年度：2015
卷号：44
期号：10
页码：1339-1347
语种：English
出版社：Tehran University of Medical Sciences
摘要：Background: Nephrotic syndrome is a disorder that leads to hyperlipidemia. L-carnitine and genistein can effect on lipid metabolism and the syndrome. In the present study, we have delved into the separate and the twin-effects of L-carnitine and genistein on the gene expressions of HMG-COA reductase and LDL receptor in experimental nephrotic syndrome.
Methods: In this controlled experimental study, 50 male Sprague–Dawley rats were randomly divided into five groups: NC (normal-control), PC (patient-control), LC (L-carnitine), G (genistein), LCG (L-carnitine-genistein). Adri-amycin was used for inducing nephrotic syndrome and the spot urine samples and urine protein-to-creatinine ratio were measured. Hepatocytic RNA was extracted and real-time PCR was used for HMG-COA Reductase and LDL receptor gene Expression measurement.
Results: The final weight of the patients groups were lower than the NC group (P=0.001), and weight gain of the NC group was higher than the other groups (P<0.001). The proteinuria and urine protein-to-creatinine ratio showed sig-nificant differences between PC group and LC, G and LCG groups at week 7 (P<0.001). The expression of HMG-COA Reductase mRNA down regulated in LC, G and LCG groups in comparison with PC group (P<0.001). ?CT of LDLr mRNA showed significant differences between the PC group and the other patient groups (P<0.001).
Conclusion: This study shows a significant decreasing (P<0.001) and non-significant increasing trend in HMG-COA Reductase and LDLr gene expression, respectively, and synergistic effect of L-carnitine and genistein on these genes in experimental nephrotic syndrome.
关键词：GENE; GENISTEIN; L-CARNITINE; LDL RECEPTOR; NEPHROTIC SYNDROME