文章基本信息

标题：In vitro studies on Vitex negundo , a potent medicinal plant
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作者：Rameshwar Groach ; Kuldeep Yadav ; Narender Singh 等
期刊名称：Environmental and Experimental Biology
印刷版ISSN：1691-8088
出版年度：2014
卷号：12
期号：4
页码：149-153
出版社：University of Latvia
摘要：An efficient plant regeneration protocol for in vitro propagation of Vitex negundo L., an important medicinal plant, was developed using mature nodal explants. Multiple shoot formation was significantly influenced by growth regulators, carbon source and type of solidifying agent used. Among the various combinations used, Murashige and Skoog medium with 2.0 mg L –1 6-benzylaminopurine + 0.5 mg L –1 α-naphthalene acetic acid, containing 25% sugarcane juice and 0.8% agar, exhibited highest bud-forming capacity (93.3%) with maximum number of formed shoots per explant (4.14). Maximum root induction response (66.6 %) was observed on Murashige and Skoog half strength semisolid medium supplemented with 1.0 mg L –1 indole-3-butyric acid. The regenerated plantlets were successfully transferred to pots containing sterilized soil and sand (3:1) mixture and acclimatized with >70% survival rate under field conditions
关键词：carbon source; solidifying agents; micropropagation; multiple shoots; Vitex negundo